The purpose of this study was to detect the IgG level of goats treated with immunogen thoracic of Musca domestica (ITMd). We reared twelve young goats aged two months old under extensive treatments, divided into two equal groups for a comparison experimental design. The animals in the first group (R0) served as the control without immunogen injection, and the second group (R1) was the treatment group receiving a subcutaneous injection of 10 mL of thoracic antigens per head. The parameter observed was IgG (immunoglobulin-G) antibody serum level. The quantification of goat immunoglobulins was carried out using a single radial immuno-diffusion (SRID) technique. The data obtained from the two groups were analyzed with a t-student test. The results showed that the total IgG antibody serum of goats in the treatment group was significantly higher than that of the control group (P< 0.05). This difference may be due to the thoracic M. domestica immunogen response which increased antibody synthesis of experiment goats. We concluded that the treatment of thoracic immunogen of M. domestica at a level of 10 µL injected subcutaneously could significantly increase IgG antibodies in goat blood which were detected using a single radial immuno-diffusion method.
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Adamski Z, SA Bufo, S Chowański, P Falabella, J Lubawy, P Marciniak, J Pacholska-Bogalska, R Salvia, L Scrano, M Słocińska, and M Spochacz. 2019. Beetles as model organisms in physiological, biomedical and environmental studies–a review. Frontiers in physiology. 10:319.
Ariantini B, H Ratnani, EM Luqman and P Hastutiek. 2019. Antibody Titers in The Sheep which were Immunated Antigen of Whole Protein from Third Instar Larvae Musca domestica. IOP Conference Series: Earth and Environmental Science, Volume 217, Number 1. doi:10.1088/1755-1315/217/1/012022
Breijo M, E Esteves, B Bizzarro, PG Lara, JB Assis, S Rocha, L Pastro, C Fernández, A Meikle, and A Sá-Nunes. 2018. Hematobin is a novel immunomodulatory protein from the saliva of the horn fly Haematobia irritans that inhibits the inflammatory response in murine macrophages. Parasites & Vectors. 11(1):435.
Chen L, J Zhang, and H Sun. 2015. Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica. BMC Complementary and Alternative Medicine. 15:427.
Cherniack EP. 2010. Bugs as Drugs, Part 1: Insects. The “New” Alternative Medicine for the 21st Century? Alternative Medicine Review 15(2):124-135.
Chernysh S and I Kozuharova. 2013. Anti-tumor activity of a peptide combining patterns of insect alloferons and mammalian immunoglobulins in naive and tumor antigen vaccinated mice. International Immunopharmacology. 17(4):1090-1093.
Chowanski S, Z Adamski, J Lubawy, P Marciniak, J Pacholska-Bogalska, M Slocinska, M Spochacz, M Szymczak, A Urbanski, K Walkowiak-Nowicka, and G Rosinski. 2017. Insect peptides–perspectives in human diseases treatment. Current Medicinal Chemistry. 24(29):3116-52.
Czopowicz M, O Szaluś‐Jordanow, M Mickiewicz, A Moroz, L Witkowski, I Markowska-Daniel, D Reczyńska, E Bagnicka, and J Kaba. 2018. Decline of maternal antibodies to small ruminant lentivirus in goat kids. Animal Science Journal. 89(9):1364-70.
Duysburgh E, L Mortgat, C Barbezange, K Dierick, N Fischer, L Heyndrickx, V Hutse, I Thomas, S Van Gucht, B Vuylsteke, and KK Ariën. 2021. Persistence of IgG response to SARS-CoV-2. The Lancet Infectious Diseases. 21(2):163-4.
Hurley WL and PK Theil. 2011. Perspectives on immunoglobulins in colostrum and milk. Nutrients. 3(4):442-74.
Kamada N, GY Chen, N Inohara, and G Núñez. 2013. Control of pathogens and pathobionts by the gut microbiota. Nature immunology. 14(7):685-90.
Luecke S, KM Sheu, and A Hoffmann. 2021. Stimulus-specific response in innate immunity: Multiyered regulatory circuits. In Immunity. 54 (9): 1915-1932. DOI: https://doi.org/10.1016/j.immuni.2021.08.018
Rumokoy L, Assa G, Moningkey S, Manangkot H, Sumolang C, Toar WL. 2020. Thoraxial Antigen-G of House Fly Musca domestica (Muscidae: Diptera) on Serum Immunoglobulin Level of Goats. In International Conference and the 10th Congress of the Entomological Society of Indonesia (ICCESI 2019) 2020 May 18 (pp. 165-168). Atlantis Press. https://dx.doi.org/10.2991/absr.k.200513.029.
Rumokoy L, S Adiani, GJV Assa, WL Toar, JL Aban. 2017. Entomology contribution in animal immunity: Determination of the crude thoraxial glandular protein extract of Stomoxys calcitrans as an antibody production enhancer in young horses. Journal of Entomological and Acarological Research. 49 (3):140-143. DOI https://doi.org/10.4081/jear.2017.7074
Rumokoy LJ and WL Toar. 2014. The equine colostrums of milk treatment against pathogenic agent. Scientific Papers Series D. Animal Sciencevol. 1(52):174-7.
Toar WL, C Kaunang, IM Untu, L Rumokoy, H Kiroh. 2017. The empowerment of crude extract antigens-G of insect on goat immunity enhancement: An entomology contribution in animal husbandry. Scientific Papers: Series D, Animal Science. 60:271-273.
Toar WL, L Rumokoy, IM Untu, and G Assa. 2019. Insect Crude Thoraxial Antigen-G Extracted from Apis mellifera to Enhance Serum Immunoglobulin of Goats: An Entomology Contribution in Animal Science. Animal Production. 20(2): 133-138.
Zar JH. 2010. Biostatistical analysis, Fifth Edition. Pearson Prentice Hall. New Jersey. p 130-142.