Swine cattle have high economic advantages and social values in Balinese society. This study aimed to determine the effectiveness of Enterotoxigenic Escherichia coli (ETEC) vaccine in gestating sows. We used 26 gestating sows aged 1.5 years old at their last trimester assigning 13 sows in the control group and 13 sows in treatment group to receive multivalent ETEC vaccine twice: on the 70-75 days of gestation and a booster on 100-105 days of gestation. Sows with the vaccine treatment divided into 3 group that groups A was treated antifimbrial K41, groups B was antifimbrial 987P, while in group C was Antiimbrial K88. The changes in temperature were observed from one day before to 4 days after vaccination in a randomized block design. To determine the antibody formed, blood samples were taken one and two weeks after the first vaccine and five weeks after the booster. The blood sample was analyzed by the Enzyme Linked Immunosorbent Assay (ELISA) technique. The rates of diarrhea and postnatal mortality were also observed. The results showed that the antibody titer level increased significantly in the fifth week in groups A with titer 1,94 ±0,22 and B with titer 1,98 ± 0,30 , while the number of diarrhea incidents was 0.9% compared to control 34% with a mortality rate of 0%. In conclusion, this multivalent ETEC vaccine is safe to use and has been shown to be effective for ETEC cases in swine.
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Cox, E., V. Melkebeek, B. Devriendt, B. Goddeeris, and D. Vanrompay. 2014. Vaccines against enteric E. coli infections in animals. Pathogenic Escherichia Coli: Molecular and Cellular Microbiology, 255-270.
Fairbrother, J. M., and Nadeau, É. 2019. Colibacillosis. Diseases of swine, 807-834.
Hashish, E.A., C. Zhang, X. Ruan, D.E. Knudsen, C.C. Chase, R.E. Isaacson, ... and W. Zhang. 2013. A multiepitope fusion antigen elicits neutralizing antibodies against enterotoxigenic Escherichia coli and homologous bovine viral diarrhea virus in vitro. Clinical and Vaccine Immunology, 20(7), 1076-1083.
Hur, J. and J.H. Lee. 2012. Comparative evaluation of a vaccine candidate expressing enterotoxigenic Escherichia coli (ETEC) adhesins for colibacillosis with a commercial vaccine using a pig model. Vaccine, 30(26), 3829-3833.
Hur, J. and J.H. Lee. 2012. Development of a novel live vaccine delivering enterotoxigenic Escherichia coli fimbrial antigens to prevent post-weaning diarrhea in piglets. Veterinary immunology and immunopathology, 146(3-4), 283-288.
Hur, J. and J.H. Lee. 2013. Protection against neonatal Escherichia coli diarrhea by vaccination of sows with a novel multivalent vaccine candidate expressing E. coli adhesins associated with neonatal pig colibacillosis. Research in Veterinary Science, 94(2), 198–204. https://doi.org/10.1016/j.rvsc.2012.08.004
Melkebeek, V., B.M. Goddeeris, and E. Cox. 2013. ETEC vaccination in pigs. Veterinary immunology
and immunopathology, 152(1-2), 37-42.
Nagy L.K., T. MacKenzie, K.R. Painter. Protection of the nursing pig against experimentally induced enteric colibacillosis by vaccination of dam with fimbrial antigens of E coli (K88, K99 and 987P). Vet Rec. 1985;117(16):408-413. doi:10.1136/vr.117.16.408
Parwati, I.A., N. Suyasa, and A.S.J. Utami. 2019. Level Of Swine Productivity On The Swine Development Program In Gianyar Region. In Proceeding of the International Conference on Food and Agriculture (Vol. 2, No. 1).
Priyadi A and L. Natalia. 2005. Bakteri Penyebab Diare pada Sapi dan Kerbau di Indonesia. Seminar Nasional Teknologi Peternakan dan Veteriner 2005. http://peternakan.litbang.deptan.go.id Download:16 Juli 2009
Supar, R.G. Hirst, and B.E. Patten. 1990. Antimicrobial drug resistance in enterotoxigenic Escherichia coli K88, K99, F41, and 987P isolated from piglets in Indonesia. Penyakit Hewan 22: 13-19.
Supar. 1996. Studi Kolibasilosis Pada Anak Sapi Perah dan Deteksi Escherichia coli K99, F 41 dan K-99F41 dalam Prosiding Temu Ilmiah Nasional Bidang Veteriner. Maret 1996. p148-155
Steffen, R., Z.D. Jiang, M.L. Gracias Garcia, P. Araujo, M. Stiess, T. Nacak, ... and H.L. DuPont. 2018. Rifamycin SV-MMX® for treatment of travellers’ diarrhea: equally effective as ciprofloxacin and not associated with the acquisition of multi-drug resistant bacteria.
Takeyama, N., Y. Yuki, D. Tokuhara, K. Oroku, M. Mejima, S. Kurokawa, ... and H. Kiyono. 2015. Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs. Vaccine, 33(39), 5204-5211.
Yasa, R., Y. A. Parwati, S. Guntoro, N. Suyasa, and I.K. Saka. 2000. The effect of enzyme on pig fattening. In Prosiding seminar pengembangan teknologi pertanian ramah lingkungan, Denpasar (Indonesia), 8-9 Mar 2000. Pusat Penelitian Sosial Ekonomi.
Yasa. R.I.M. and I.A. Parvati. 2001. Disease Control kolibasilosis with multivalent ETEC vaccine. Papers of the National Seminar on Economic Cooperation Center for Agricultural-Bogor Institute for Agricultural Technology-Bali.
Wulansari, R., A. Esfandiari, I.W.T. Wibawan, and S. Murtini. 2016. Kinerja Kesehatan Sapi Neonatus yang Diberi Kolostrum dari Induk Sapi yang Divaksin Escherichia coli. Acta VETERINARIA Indonesiana, 4(1), 19-26.