Effects of Toxin Binder Supplementation via Drinking Water on The Growth Performance of Broiler Chickens


Dixie GE Mendoza
Listya Purnamasari
Joseph P Olarve
Joseph F dela Cruz


A four-week feeding trial was conducted to determine the effect of liquid toxin binder supplementation on the production performance of broiler chickens. A total of 400 seven-day-old Cobb broiler chicks were randomly distributed to four treatments following a Complete Randomized Design (CRD). The treatment groups consisted of the control group, continuous liquid toxin binder supplementation, reduced feed toxin binder inclusion, continuous liquid toxin binder supplementation, and intermittent liquid toxin binder supplementation. Each treatment was replicated four times with 25 chicks per replicate. The feeding trial was conducted from the age of 8th to 35th days of age. The acidifier used was a blend of activated charcoal, selenium, vitamin E, probiotics, prebiotics, mannan oligosaccharides, and bacterial cell wall components. The results showed significant effects (P<0.05) on the broilers' body weight gain, average daily gain, and feed efficiency from 8 to 14 days of age. Income over feed, chick, and medication cost of broilers was increased with continuous supplementation of liquid toxin binder and reduced feed toxin binder. This study indicates that continuous supplementation of liquid toxin binder and reducing the feed toxin binder is economically more advantageous to use in broiler production.


How to Cite
Mendoza, D. G., Purnamasari, L., Olarve, J. P., & dela Cruz, J. F. (2022). Effects of Toxin Binder Supplementation via Drinking Water on The Growth Performance of Broiler Chickens. ANIMAL PRODUCTION, 24(3), 142-149. https://doi.org/10.20884/1.jap.2022.24.3.168


Adegbeye, MJ, PRK Reddy, CA Chilaka, OB Balogun, MMMY Elghandour, RR Rivas-Caceres, and AZM Salem. 2020. Mycotoxin toxicity and residue in animal products: Prevalence, consumer exposure and reduction strategies – A review. Toxicon 117: 96 – 108. https://doi.org/10.1016/j.toxicon.2020.01.007
Ardiansyah, W, O Sjofjan, E Widodo, S Suyadi, and DN Adli. 2022. Effects of combinations of α-Lactobacillus sp. and Curcuma longa flour on production, egg quality, and intestinal profile of Mojosari ducks. Advances Animal Veterinary Science. Vol 10(8): 1668-1677.
Alpsoy, L, and ME Yalyac. 2011. Chapter twelve - Key Roles of Vitamins A, C, and E in Aflatoxin B1-Induced Oxidative Stress. Vitamins & Hormones 86 287 – 305. https://doi.org/10.1016/B978-0-12-386960-9.00012-5
Bobade, SP, AN Sarag, DH Rekhate, AP Dhok and SV Joge. 2009. Efficacy of Vitamin E and Selenium in Growth Performance of Broilers. Veterinary World. Vol 2 (1): 20 – 21. http://dx.doi.org/10.5455/vetworld.2009.20-21
Coppock, RW, RG Christian, and BJ Jacobsen. 2018. Chapter 69 – Aflatoxins. Veterinary Toxicology (Third Edition), 983 – 994. https://doi.org/10.1016/B978-0-12-811410-0.00069-6
Dazuk, V, MM Boiago, G Rolim, A Paravisi, PM Copetti, BF Bissacotti, VM Morsch, M Vedovatto, FL Gazoni, F Matte, EM Gloria, AS Da Silva. 2020. Laying hens fed mycotoxin-contaminated feed produced by Fusarium fungi (T-2 toxin and fumonisin B1) and Saccharomyces cerevisiae lysate: Impacts on poultry health, productive efficiency, and egg quality. Microbial Pathogenesis, 149, https://doi.org/10.1016/j.micpath.2020.104517
de Freitas, CH, CL Goncalves, and P da Silva Nascente. 2018. Aflatoxins B1 and M1: risks related to milk produced in Brazil. Annals of Microbiology 68:793 – 802. https://doi.org/10.1007/s13213-018-1395-2
Devegowda, G and TNK Murthy. 2005. Mycotoxin: their effects in poultry and some practical solutions. In Duerte D (ed.). The Mycotoxin Blue Book. United Kingdom: Nottingham University Press.
Dvorska, JE, AC Pappas, F Karadas, BK Speake, and PF Surai. 2007. Protective effect of modified glucomannans and organic selenium against antioxidant depletion in the chicken liver due to T-2 toxin-contaminated feed consumption. Comparative Biochemistry and Physiology 145. 582 – 587. https://doi.org/10.1016/j.cbpc.2007.02.005
Guo, Y, Y Zhang, C Wei, Q Ma, C Ji, J Zhang, and L Zhao. 2019. Efficacy of Bacillus subtilis ANSB060 Biodegradation Product for the Reduction of the Milk Aflatoxin M1 Content of Dairy Cows Exposed to Aflatoxin B1. Toxins. Vol 11(3): 1-10 https://doi.org/10.3390/toxins11030161
Hassan, AA, RM Sayed-Elahl, NH Oraby, and AMA El-Hamaky. 2020. Chapter 11 - Metal nanoparticles for management of mycotoxigenic fungi and mycotoxicosis diseases of animals and poultry. Nanomycotoxicology, 251-269 https://doi.org/10.1016/B978-0-12-817998-7.00011-2
Huwig, A, S Freimund, O Kappeli, and H Dutler. 2001. Mycotoxin detoxication of animal feed by different adsorbents. Toxicology Letters 122. 179 – 188. https://doi.org/10.1016/s0378-4274(01)00360-5
Isabelle, MB, JK Tuitoek, AM King’ori, and MA Obonyo. 2020. The Effect of Fermented Aflatoxins Contaminated Feed on Digestibility and Performance of Broiler Chickens. Animal Production. Vol 22(1): 55-60
Khan, CM. 2010. The Merck Veterinary Manual. 10th ed. New Jersey, USA: Merck & Co., Inc.
Koppenol, A, BCB Beirao, M Ingberman, LP Caron. 2019. Measuring Peripheral and Some Mucosal Immune Cells to Better Understand Immunomodulation by T-2 Toxin in Broilers. Journal of Applied Poultry Research. Vol 28(4): 837 – 845. https://doi.org/10.3382/japr/pfz040
Ma, R, L Zhang, L Meng, YT Su, WM Xie, NY Zhang, JF Dai, Y Wang, SA Rajput, DS Qi, NA Korraw, LH Sun. 2018. Individual and Combined Occurrence of Mycotoxins in Feed Ingredients and Complete Feeds in China. Toxins 10(3), https://doi.org/10.3390/toxins10030113
Maki, CR, S Haney, M Wang, SH Ward, BJ Rude, RH Bailey, RB Harvey, and TD Phillips. 2017. Calcium montmorillonite clay for the reduction of aflatoxin residues in milk and dairy products. Journal of Dairy & Veterinary Science. Vol 2: 1-11 http://dx.doi.org/10.19080/JDVS.2017.02.555587
Mehdi, A and G Hasan. 2012. Immune Response of broiler chicks fed yeast-derived mannan oligosaccharides and humate against Newcastle disease. World Applied Sciences Journal. Vol 18 (6): 779-785. http://dx.doi.org/10.5829/idosi.wasj.2012.18.06.3362
Mubarak, A, A Rashid, IA Khan, and A Hussain. 2009. Effect of Vitamin E and Selenium as immunomodulators in induced aflatoxicosis in broiler birds. Pakistan Journal of Life and Social Science. Vol 7 (1). 31 – 34.
Murugesan, GR, DR Ledoux, K Naehrer, F Berthiller, TJ Applegate, B Grenier, TD Phillips, and G Schatzmayr. 2015. Prevalence and effects of mycotoxins on poultry health and performance, and recent development in mycotoxin counteracting strategies. Poultry Science. Vol 94(6):1298 – 1315. https://doi.org/10.3382/ps/pev075
Panzhanivel, N and C Balachandran. 2014. Alleviative Effect of Mannan Oligosaccharide against penicillic acid toxicity in broiler chickens. International Journal of Life Science and Pharma Research. 4 (3): 13 -27.
Pitt, JI, and JD Miller. 2017. A Concise History of Mycotoxin Research. Journal of Agricultural and Food Chemistry. Vol 65(33): 7021- 7033. https://doi.org/10.1021/acs.jafc.6b04494
Plumb, DC. 2008. Veterinary Drug Handbook. 6th ed. Ames IA: Blackwell Publishing.
Plumb, K. 2004. Clinical Veterinary Toxicology. Missouri, Mosby Inc.
Purnamasari, L, A Agus, and CT Noviandi. 2019. Effects of Methionine-Cysteine Amino Acid Supplementations in the Aflatoxin B1 Contaminated Diet on Broiler Production Performance. Bulletin of Animal Science. Vol 43(4):231 – 236. https://doi.org/10.21059/buletinpeternak.v43i4.31150
Purnamasari, L, A Agus, and CT Noviandi. 2020. Pengaruh pemberian asam amino metionin-sistin pada pakan yang terkontaminasi aflatoxin b1 terhadap mortalitas dan kinerja organ dalam ayam broiler. Jurnal Ilmu Ternak. Vol 20(1): 46 – 55. https://doi.org/10.24198/jit.v20i1.28582
Sjofjan, O, DN Adli, MH Natsir, YF Nuningtyas, TS Wardani, I Sholichatunnisa, SN Ulpah, O Firmansyah. 2021. Effect of dietary modified-banana-tuber meal substituting dietary corn on growth performance, carcass trait and dietary-nutrients digestibility of coloured-feather hybrid duck. J. Ilmu Ternak dan Veteriner. Vol 26(1):39-48.
Sjofjan, O and DN Adli. 2021. The effect of replacing fish meal with fermented sago larvae (FSL) on broiler performance. Livestock Research Rural Development 33: 2.
Stanley, VG, R Ojo, S Woldensenbet and DH Hutchinson. 1993. The use of Saccharomyces cerevisiae to suppress the effect of aflatoxicosis in broiler chicks. Poultry Science. 72, 1867-1872 https://doi.org/10.3382/ps.0721867
Upton, JR, FW Edens, and PR Ferket, 2008. Selenium Yeast Effect on Broiler Performance. International Journal of Poultry Science. Vol 7(8): 798 – 805. http://dx.doi.org/10.3923/ijps.2008.798.805
Wang, L, Q Zhang, Z Yan, Y Tan, R Zhu, D Yu, H Yang and A Wu. 2018. Occurrence and Quantitative Risk Assessment of Twelve Mycotoxins in Eggs and Chicken Tissues in China. Toxins. Vol 10(11): 1 – 18. https://doi.org/10.3390/toxins10110477
Zaki, MM, SA El-Midany, HM Shaheen, and L Rizzi. 2012. Mycotoxins in animals: Occurrence, effects, prevention, and management. Journal of Toxicology and Environmental Health Sciences. Vol 4(1): 13-28. https://doi.org/10.5897/JTEHS11.072