Indigofera zollingeriana Leaf Extract Reduces Sheep Rumen Methane Production in Vitro

  • FM Suhartati University of Jenderal Soedirman
Keywords: Indigofera zollingeriana, leaf extract, protozoa, methane, bacteria

Abstract

An experimental study aimed at assessing the reduction of gas methane production in   thin-tailed sheep through the use of Indigofera zollingeriana leaf extract was conducted from May to September 2019 at Animal Nutrition and Feed Laboratory, University of Jenderal Soedirman Purwokerto. A One Way Classification of Completely Randomized Design (CRD) experiment was employed. The treatment included Indigofera zollingeriana leaf extract with levels of 0%, 0.40%, and 0.80% of feed dry matter and each treatment was repeated six times so that there were 18 experimental units. The feed provided consisted of 60% concentrate and 40% ammoniated rice straw. The concentrate consisted of coconut cake and rice bran with a ratio composition of 1:2. The material used was rumen fluid obtained from three thin-tailed sheep, sampled from the Sokaraja slaughterhouse immediately after the sheep were slaughtered. In vitro incubation was carried out for four hours. The variables measured included protozoan population, methane gas production and bacterial populations. The data obtained were then analyzed using analysis of variance and continued with the Orthogonal Polynomial test. The use of 0.80% Indigofera zollingeriana leaf extract can reduce the protozoan populations, methane gas production and can increase the bacterial populations of sheep rumen fluid.

References

Diaz, A., M. Avendano and E. Escobar. 1993. Evaluating of Sapindus saponaria as a defaunating agent and its effects on different ruminal digestion parameters. Livest. Res. Rural Dev. 5:1-6.

Herawati, D.A. dan Adang, A.W. 2010. Pengaruh pretreatmen jerami padi pada produksi biogas dari jerami padi dan sampah sayur hijau secara batch. Jurnal Rekayasa Proses 4 (1):25-29.

Hess, H. D., R. A. Beuret, M. Lötscher, I. K.Hindrichsen, A. Machmüller, J. E. Carulla, C. E. Lascano and M. Kreuzer. 2004. Ruminal fermentation, methanogenesis and nitrogen utilization of sheep receiving tropical grass hayconcentrate diets offered with Sapindus saponaria fruits and Cratylia argentea foliage. J. of Anim. Sci. 79:177-189.

Jayanegara A. dan A. Sofyan, 2008. Penentuan aktivitas biologis tanin secara in vitro menggunakan Hohenheim gas test dengan polietilen glikol sebagai determinan. Media Peterenakan 31 (1) : 44-52

Kreuzer M, and Soliva C.R. 2008. Nutrition: key to methane itigation in ruminants. Proc.Soc. Nutr. Physiol. 17: 168-171.

McAllisterl, t.a., E. K. Okine, G. W. Mathison, and K.J. Cheng. 1996. Dietary, environmental and microbiological aspects of methane Production in ruminants. Canadian Journal of Animal Science. 76: 231-243.

Prasetiyono, B.W.H.E., Suryahadi, T. Tohormat dan R. Syarief. 2007. Strategi suplementasi protein ransum sapi potong berbasis jerami dan dedak padi. Media Peternakan 30 (3):207-217.

Santoso, B dan B.Tj. Hariadi. 2007. Pengaruh Suplementasi Acacia mangium Willd pada Pennisetum purpureum terhadap Karakteristik Fermentasi dan Produksi Gas metana in-vitro. Jurnal Media Peternakan. 30 (2) :106-113.

Steel, R.G.D. and J.H. Torrie. 1993 Prinsip dan Prosedur Statistika. Suatu Pendekatan Biometrik. Edisi kedua. Diterjemahkan oleh Sumantri, B. PT Gramedia Pustaka Utama, Jakarta

Suryapratama, W., F. M. Suhartati, S. Rahayu. 2018. Penggunaan ekstrak daun kelor (Moringa oleifera) sebagai upaya untuk menurunkan produksi gas metan (CH4) domba. Laporan Penelitian Riset Unggulan Unsoed Terapan (tidak dipublikasi)

Susanti, S dan E. Marhaeniyanto. 2014. Kadar saponin daun tanaman yang berpotensi menekan gas metana secara in-vitro. Buana Sains (14) 1: 29-38.

Tamminga, S. 1982. Energy-proteinrelationships in ruminant feeding similarities and differences between rumen fermentation and postruminal utilization. In: Protein contribution feedstuffs for ruminant: applications of feed formulation. E. L. Miller and I.H. Pike (Editors). International Association of fish meal manufacture. Butterworth Scientific, London.

Tilley, J. M. A and R. A. Terry. 1963. Thr relationship between the soluble constituen herbage and their dry matter digestibility. Journal British Feed Science. 18:104-111.

US Environment Protection Agency. 1994. International anthropogenic methane emission: estimates for 1990. EPA 230-R93-010. Washington DC: Office of Policy, Planning and Evaluation

Wang, Y., T. A. McAllister, L. J. Yanke, Z. J. Xu, P. R. Cheeke and K. J. Cheng. 2000. In-vitro effects of steroidal saponins from Yucca schidigera extract on rumen microbes. J. Appl. Microbiol. 88:887-896

Published
2020-07-03
How to Cite
Suhartati, F. (2020). Indigofera zollingeriana Leaf Extract Reduces Sheep Rumen Methane Production in Vitro. ANIMAL PRODUCTION, 22(1), 16-23. https://doi.org/10.20884/1.jap.2020.22.1.39
Section
Articles