Protein is one of the nutrient components in milk that is related to product quality. The components of milk protein are divided into casein alpha-s1, beta, alpha-s2, kappa, and whey fractions such as alpha lactalbumin and beta lactoglobulin. There are no existing data of milk protein fraction in dairy cow and goats in Banyumas Regency. This study aimed to determine the profile in form of protein fractions of cow and goat milk in Banyumas. Milk sample from fifty cows and thirty dairy goats was taken by random sampling in some areas. The milk protein profile was identified by the technique of sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) and protein quantity prediction by software. The data obtained were analyzed statistically by Mann-Whitney between cows and goats. The results were significantly different (p<0.05) between cows and goats in molecular weight of protein alpha-S1 casein (29.66 vs 33.37 kDa), alpha-S2 (27.76 vs 29.49 kDa), beta (24 , 48 vs 25.59 kDa) and beta lactoglobulin (15.75 vs 15.97 kDa). The quantity of casein alpha-S1 (7.88 vs 4.16 g/l), alpha-S2 (1.31 vs. 4.02 g/l), beta (8.74 vs 14.24 g/l), kappa (2.41 vs. 4.28 g/l) and alpha lactalbumin (0.91 vs 0.7 g / l) was significantly different (p <0.05) between cow's and goat's milk, respectively. In conclusion, milk protein profile of cows and goats in Banyumas Regency is different.
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Caroli, A.M., S. Chessa, and G.J. Erhardt. 2009. Invited review: Milk protein polymorphisms in cattle: Effect on animal breeding and human nutrition. J. Dairy Sci. 92 :5335–5352. DOI 10.3168/jds.2009-2461.
Costa, F.F., M.A.V.P. Brito, M.A.M. Furtado, M.F. Martins, M.A.L de Oliveira, P.M. de Castro Barra, L.A. Garridoc and A.S. de Oliveira dos Santos. 2013. Microfluidic chip electrophoresis investigation of major milk proteins: study of buffer effects and quantitative approaching. Analytical Methods. DOI: 10.1039/c3ay41706a
Damian, J.P., I. Saccji, S. Reginesi, D. De Lima and J. Bermudez. 2008. Cheese yield, casein fractions and major components of milk of Saanen and Anglo-Nubian dairy goats. Arq. Bras. Med. Vet. Zootec 60(6).
Kumar, A., P.K. Rout and B.P. Mohanty. 2013. identification of milk protein polymorphism in indian goats by 2D gel electrophoresis. J. Of Proteomics and Bioinformatics 6. DOI 10.4172/jpb.1000252.
Li, K., Z. Chen, F. Duan, J. Liang and K. Wu. 2010. Quantification of tear proteins by SDS-PAGE with an internal standard protein: A new method with special reference to small volume tears. Graefe's Archive for Clinical and Experimental Ophthalmology 248(6):853-862.
Masoodi, T.A. and G. Shafi. 2010. Analysis of casein alpha S1 & S2 proteins from different mammalian species. Bioinformation 4(9):430-435.
Moatsou, G., E. Moschopoulou, D. Molle, V. Gagnaire, I. Kandarakis and J. Leonil. 2008. Comparative study of the protein fraction of goat milk from the Indigenous Greek breed and from international breeds. Food Chemistry 106(2):509-520.
Pemkab Banyumas. 2018. Banyumas Regency Government: Geographical Location. Online : https://www.banyumaskab.go.id/page/307/letak-geografis (Visited 1 December 2018).
Prizenberg, E.M., K. Gutcher, S.M. Chessa, A. Caroli and G. Erhardt. 2005. Caprine K-casein (CSN3) polymorphism : New development in molecular knowledge. J. Dairy Sci. 88:1490- 1498.
Sudjatmogo, H.S. Widodo and Mukson. 2014. Kajian Tentang Kualitas, Pola Distribusi dan Harga Susu di Kabupaten Banyumas dan Kabupaten Semarang (Study of quality, distribution pattern and price of milk in Banyumas regency and Semarang regency) In Prosiding Seminar Nasional Ruminansia. Semarang. pp : 224-226.
Widodo, H.S., T.W. Murti, A. Agus dan W. Widodo. 2018. Mengidentifikasi peptida bioaktif Angiotensin Converting Enzyme-inhibitor (ACEi) dari kasein β susu kambing dengan polimorfismenya melalui teknik in silico (Identification of angiotensin converting enzyme-inhibitor (ACEi) Bioactive peptide from goat milk β-Casein with it's polymorphism by in silico technique). Jurnal Aplikasi Teknologi Pangan 7(4). https://doi.org/10.17728 /jatp. 3008.