Antigen-G of Larva Serum of Musca domestica as an Immunoglobulin Production Promoter in Goats under an Organic Maintenance

  • Laurentius Rumokoy Sam Ratulangi University
  • Hengky Kiroh Sam Ratulangi University
  • Ivonne Maria Untu Sam Ratulangi University
  • Geertruida Assa Sam Ratulangi University
  • Santie Turangan Sam Ratulangi University
  • Wisje Lusia Toar Sam Ratulangi University
Keywords: insect, Musca domestica, antigen, antibody, goats

Abstract

This experiment aimed to reveal the effect of serum G-antigen of M. domesticainsect larvae (LAS) as promoter antigen on serum immunoglobulin production in organically managed goat livestock. This study used 12 local goats which were divided into two groups: as a control group and treated group receiving treatment. Insect rearing was conducted to obtain the larvae, the antigen-G was then extracted from the larvae to be used as promoter antigen to enhance the serum antibody production which was subcutaneously immunized in experimental goats and incubated for a period of 14 days. Blood collection of 2.5 ml was taken through the jugular vein and then carried out a quantification of serum protein level indicating the antibody proportion of goats. The data of the LSA extract proportion level were statistically analyzed with t-test, and the quality classification level of serum immunoglobulin of animal groups were statistically analysed. The results showed that the serum of animal treated with LSA of M. domesticaresulted in a higher level of immunoglobulin (P <0.01) compared to the control. We conclude that the antigen-g substance (LSA) could support the efforts to improve the production of organic goat livestock by increasing the total level of antibodies circulating in the blood.

References

Ai H, Wang F, Zhang N, Zhang L, Lei C. 2013. Antiviral, immunomodulatory, andfree radical scavenging activities of a protein-enriched fraction from thelarvae of the housefly, Musca domestica. J Insect Sci 13:112

Ameri M, X Wang, MJ Wilkerson, MR Kanost, and AB Broce. 2008. An Immunoglobulin Binding Protein (Antigen 5) of the Stable Fly (Diptera: Muscidae) Salivary Gland Stimulates Bovine Immune Responses. J Med Entomol. 45(1): 94–101. doi: 10.1603/0022-2585(2008)45[94:aibpao]2.0.co.

Ariantini B, H Ratnani, EM Luqman and P Hastutiek. 2019. Antibody Titers in The Sheep which were Immunated Antigen of Whole Protein from Third Instar Larvae Musca domestica. IOP Conference Series: Earth and Environmental Science, Volume 217, Number 1. doi:10.1088/1755-1315/217/1/012022

Chen L, Zhang J, and H Sun. 2015. Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica. BMC Complementary and Alternative Medicine 15:427

Cherniack EP. 2010. Bugs as Drugs, Part 1: Insects. The “New” Alternative Medicine for the 21st Century? Alternative Medicine Review 15(2):124-135.

Keiding J and K Arevad. 1964. Procedure and equipment for rearing a large number of house fly strains. Bull.Org.mond.Santé. 31:527-528.

Rumokoy L, Posangi J, Toar WL, and J Lopez-Aban. 2018. An expectation of bio-resource function against parasite infection on animal health. Sci. Papers. Series D. Animal Science 61(1):216-219. http://animalsciencejournal.usamv.ro/pdf/2018/issue_1/Art38.pdf.

Rumokoy L, S Adiani, GJV Assa, WL Toar, JL Aban. 2017. Entomology contribution in animal immunity: Determination of the crude thoraxial glandular protein extract of Stomoxys calcitrans as an antibody production enhancer in young horses. 49 (3):140-143. DOI https://doi.org/10.4081/jear.2017.7074

Rumokoy L and WL Toar. 2014. The Equine colostrums of milk treatment against pathogenic agent. Scientific Papers. Series D. Animal Science 57:174-177.

Toar WL, C Kaunang, IM Untu, L Rumokoy, H Kiroh. 2017. The empowerment of crude extract antigens-G of insect on goat immunity enhancement: An entomology contribution in animal husbandry. Scientific Papers: Series D, Animal Science 60:271-273.

Toar WL, M Tulung, V Memah, E Pudjihastuti, Rumokoy L, Posangi J, and IM Untu. 2018 The Presence of insects in animal farm in North Sulawesi. Scientific Papers. Series D. Animal Science 61(1):220-224.

Toar WL, L Rumokoy, IM Untu, G Assa. 2018. Insect Crude Thoraxial Antigen-G Extracted from Apis mellifera to Enhance Serum Immunoglobulin of Goats: An Entomology Contribution. Animal Science. Animal Production. 20(2):133-138. http://dx.doi.org/10.20884/1.jap.2018.20.2.608.

Toar WL, LJM Rumokoy, E Pudjihastuti, H Manangkot, B Bagau and IM Untu. 2019. Effect of Supplementation of Combination of Curcuma and BSF Maggot Meal in Rations on Accumulative Weight of Native Chickens. IOP Conference Series: Earth and Environmental Science, Volume 372, conference 1. doi:10.1088/1755-1315/372/1/012009.

Zar JH. 1996 Biostatistical Analysis. 3rd Edition, Prentice Hall, Inc.
Published
2020-07-06
How to Cite
Rumokoy, L., Kiroh, H., Untu, I. M., Assa, G., Turangan, S., & Toar, W. L. (2020). Antigen-G of Larva Serum of Musca domestica as an Immunoglobulin Production Promoter in Goats under an Organic Maintenance. ANIMAL PRODUCTION, 22(1), 31-36. https://doi.org/10.20884/1.jap.2020.22.1.36
Section
Articles