Feed Intake, Blood Parameters, Digestibility and Live Weight Gain of Male Bali Cattle (Bos javanicus) Fed Ammoniation Rice Straw Supplemented by Waru (Hibiscus tiliaceus) Flower Extracts


Sri Rahayu
Veven Raymexen Bonat
Muhamad Bata


The objective of this study was to evaluate the effect of Hibiscus tiliaceus flower extracts supplementation in concentrate on performance, nutrient digestibility and blood metabolites of Bali cattle. Sixty of male Bali cattle averaging 225 ± 09.15 kg initial body weight were used in this study. They were divided into two groups. The first group (as control) was cattle fed ammoniated rice straw and concentrate (R1) and the second group was fed as the same as the control group, but concentrate was supplemented with H. tiliaceus flower extract at 200 ppm/kg of dry matter (R2). Variables measured were dry matter digestibility (DMD), organic matter digestibility (OMD), neutral detergent fiber digestibility (NDFD), and acid detergent fiber (ADFD) using total collection method. Other variables were blood glucose and urea, average daily gain (ADG), feed conversion (FC) dan feed efficiency (FE). T-test showed that DMD, OMD, ADFD, and NDFD in the second group (R2) were higher (P<0.05) than control (R1). However, there was no significant difference (P>0.05) between performance (ADG, FC, FE) and blood parameters of urea and glucose between the groups fed diets supplemented with H. tiliaceus flower extracts and control group. There was an increase in ADG and FE on R2 (0.63 ± 0.23 kg and FE 5.54 ± 1.89) compared to R1 (0.54 ± 0.32 kg and 0.54 ± 0.32). The study concluded that supplementation of Hibiscus tiliaceus flower extracts as feed additive in the concentrate diet can increase nutrient digestibility and tend to improve the performance of Bali cattle.


How to Cite
Rahayu, S., Bonat, V. R., & Bata, M. (2021). Feed Intake, Blood Parameters, Digestibility and Live Weight Gain of Male Bali Cattle (Bos javanicus) Fed Ammoniation Rice Straw Supplemented by Waru (Hibiscus tiliaceus) Flower Extracts. ANIMAL PRODUCTION, 23(3), 171-179. https://doi.org/10.20884/1.jap.2021.23.3.12


Aazami, M.H., A.M. Tahmasbi, M.H. Ghaffari, A.A. Naserian, R. Valizadehand, A.H. Ghaffari. 2013. Effects of Saponins on Rumen Fermentation, Nutrients Digestibility, Performance, and Plasma Metabolites in Sheep and Goat Kids. Annual Review & Research in Biology 3(4): 596-607.
AOAC. 2012. Official method of analysis of AOAC International. 19th ed. Assoc. of Off.Anal. Chem., Maryland, USA.
Balcells, J., A. Aris, A. Serrano, A.R. Seradj, J. Crespo, and M. Devant. 2012. Effects of an extract of plant flavonoids (Bioflavex) on rumen fermentation and performance in heifers fed highconcentrate diets. Journal of Animal Science, vol. 90, no. 13, pp. 4975–4984. https://doi.org/10.2527/jas.2011-4955
Baraka, T.A.M. and M.A. Abdl-Rahman. 2012. In vitro evaluation of sheep rumen fermentation pattern after adding different levels of eugenol-fumaric acid combinations. Veterinary World, 5, 110-117.
Belanche, A., G. Fuente, C.J. Newbold. 2014. Study of methanogen communities associated with different rumen protozoal populations. FEMS Microbiol Ecol 90: 663–677. DOI: 10.1111/1574-6941.12423
Barham, D. and P. Trinder. 1972. An improved color reagent for the determination of blood glucose by the oxidase system. Analyst 97: 142-145.
Bata, M. and S. Rahayu. 2016. Study of Hibiscus tiliaceus Leaf Extract Carrier as Additive in the Diets for Fattening of Local Cattle (in vitro). Pakistan Journal of Nutrition 15(11): 969-974. https://doi.org/10.3923/pjn.2016.969.974.
Bata, M. and S. Rahayu. 2017. Evaluation of Bioactive Substances in Hibiscus tiliaceus and Its Potential as a Ruminant Feed Additive. Current Bioactive Compounds 13:1157-164. https://doi.org/10.2174/1573407213666170109151904
Becker, P.M., P.G. van Wikselaar, M.C.R. Franssen, R.C.H. de Vos, and R.D. Hall, J. Beekwilder. 2014. Evidence for a hydrogen-sink mechanism of (+) catechin-mediated emission reduction of the ruminant greenhouse gas methane. Metabolomics, 10, 179–189. https://doi.org/10.1007/s11306-013-0554-5
Bodas, R., N. Prieto, R. Garcia-Gonzalez, S. Andres, F.J. Giraldez, and S. Lopez. 2012. Manipulation of rumen fermentation and methane production with plant secondary metabolites. Animal Feed Science and Technology. 176(1–4):78–93. https://doi.org/10.2478/aoas-2018-0037.
Biro Pusat Statistik. 2016. Nusa Tenggara Timur Dalam Angka. Biro Pusat Statistik, Kupang-Nusa Tenggara Timur.
Chaney, A.L. and E.P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin Chem. 8:130
Chen, X.B. and M.J. Gomes. 1995. Estimation Of Microbial Protein Supply To Sheep And Cattle Based On Urinary Excretion Of Purine Derivatives: An Overview Of The Technical Details. International Resources Unit, Rowett Research Institute, Bucksburn Aberdeen, UK
Canul-Solis, A.J.R., A.T. Piñeiro-VázquezA, E.G. Briceño-Poot, et al. 2014. Effect of supplementation with saponins from Yucca schidigera on ruminal methane production by Pelibuey sheep fed Pennisetum purpureum grass. Anim. Production Sci. 54(10):1834-1839. DOI: 10.1071/AN14296
Cruz, O.T.B., M.V. Valero, F. Zawadzki, D.C. Rivaroli, R.M. Prado, B.S. Lima, I.N. Prado. 2014. Effect of glycerine and essential oils (Anacardium occidentale and Ricinus communis) on animal performance, feed efficiency and carcass characteristics of crossbred bulls finished in a feedlot system. Italian J. of Anim. Sci. 13: 790-797
Faniyi, T.O., M.K. Adewumi , Ê.R. Prates, A.S. Ayangbenro. 2016. Effect of herbs and spices (plant extracts) on rumen microbial activities: a review. PUBVET v.10, n.6, p.477-486. D.O.I.: https://doi.org/10.22256/pubvet.v10n6.477-486
Geraci, J.I., A.D. Garciarena, G.A. Gagliostro, K.A. Beauchemin, D. Colombatto. 2012. Plant extracts containing cinnamaldehyde, eugenol and capsicum oleoresin added to feedlot cattle diets: Ruminal environment, short term intake pattern and animal performance. Anim. Feed Sci. and Technol. 176: 123-130.
Goel, G., and H.P.S. Makkar. 2012. Methane mitigation from ruminants using tannins and saponins. Trop. Anim. Health Prod. 44:729–739. DOI 10.1007/s11250-011-9966-2
Greening, C., R. Geier, C. Wang, et al. 2019. Diverse hydrogen production and consumption pathways influence methane production in ruminants. The ISME Journal https://doi.org/10.1038/s41396-019-0464-2
Gunawan, A., R. Sari, Y. Parwoto and M.J. Uddin. 2011. Non genetic factors effect on reproductive performance and preweaning mortality from artificially and naturally bred in Bali Cattle. J. Indonesian Trop. Anim.Agric. 36(2) :83-90. http://doi.org/10.14710/jitaa.36.2.83-90.
Guyader, J., M. Eugène, M. Doreau, D.P. Morgavi, C. Gérard, C. Loncke, C. Martin. 2015. Tea saponin reduced methanogenesis in vitro but increased methane yield in lactating dairy cows. J. Anim. Sci. 93: 5367–5377
Hasan, S.A. and A.A. Saeed. 2012. Effect of Feeding Different Levels of Dietary Protein with High or Low Rumen Degradable: Undegradable Dietary Nitrogen on Awassi Lambs Performance 3-Selected Biochemical Parameters. KSU J. Nat. Sci. 15(3): 36-45. http://dergipark.gov.tr/download/article-file/212020
Jayanegara, A., F. Leiber, and M. Kreuzer. 2012. Meta analysis of the relationship between dietary tannin level and methane formation in ruminants from in vivo and in vitro experiments. J. of Anim. Physiol. and Anim. Nutr. 96: 365-375.
Kim, E., C.H. Kim, K.S. Min and S. Lee. 2012. Effects of plant extracts on microbial population, methane emission and ruminal fermentation characteristics in in vitro. Asian-Australasian Journal of Animal Sciences, 25, 806-811.
Kim, E.T., L.L. Guan, S.J. Lee, S.M. Lee, S.M. Lee, S.S. Lee, I.D. Lee, S.K. Lee and S.S. Lee. 2015. Effects of flavonoid-rich plan extract on In Vitro ruminal methanogenesis, microbial population and fermentation characteristics. Asian Australas. J. Anim. Sci. 28:4: 530 – 537. https://doi.org/10.5713/ajas.14.0692 ·
Kumar, S., D. Kumar, and O. Prakash. 2008. Evaluation of antioxidant potential, phenolic and flavonoid contents of Hibiscus tiliaceus flowers. Electronic Journal of Environmental Agricultural and Food Chemistry, 7: 2863–2871.https://www.researchgate.net/publication /266233970
Kumar, M, A. Kannan, R. Bhar, A. Gulati, A. Gaurav and V.K. Sharma. 2017. Nutrient intake, digestibility and performance of Gaddi kids supplemented with tea seed or tea seed saponin extract. Asian-Australas J Anim Sci Vol. 30, No. 4:486-494. https://doi.org/10.5713/ajas.16.0451
Liu, Y., T. Ma, D. Chen, N. Zhang, B. Si, K. Deng, Y. Tu and Q. Diao. 2019. Effects of Tea Saponin Supplementation on Nutrient Digestibility, Methanogenesis, and Ruminal Microbial Flora in Dorper Crossbred Ewe. Animals 9 (29): 1-11. DOI:10.3390/ani9010029
McDonald, P., R.A. Edwards, J.F.D. Greenhalgh, C.A. Morgan, L.A. Sinclair, and R.G. Wilkinson. 2010. Animal Nutrition. 7th Ed. Longman Scientific and Technical, New York.
Nasri, S., H.B. Salem, V. Vasta et al. 2011. Effect of increasing levels of Quillaja saponariaon digestion, growth and meat quality of Barbarine lamb. Anim Feed Sci Technol 164:71-78. htpps://doi.org/10.1016/j.anifeedsci.2010.12.005.
Ngadiyono, N. 2012. Growth and carcass characteristics of Sumba Ongole, Brahman cross and Australian commercial cross beef maintained intensively in various slaughter weights. [dissertation] Postgraduate Programme, Bogor Agriculture University.
Nozad, S., A.G. Ramin, G. Moghadam, S. Asri-Rezaei, A. Babapour, and S. Ramin. 2012. Relationship between blood urea, protein, creatinine, triglycerides and macromineral concentrations with the quality and quantity of milk in dairy Holstein cows. Veterinary Res. Forum. 12: 3 (1): 55 – 59
Oskoueian, E., N. Abdullah and A. Oskoueian. 2013. Effects of Flavonoids on Rumen Fermentation Activity, Methane Production, and Microbial Population. Biomed.Res. Int.: 349129. http://dx.doi.org/10.1155/2013/ 349129
Patra, A.K. and J. Saxena. 2010. A new perspective on the use of plant secondary metabolites to inhibit methanogensis in the rumen. Phytochemistry 71:1198-222. https://doi.org/10.1016/j.phytochem.2010.05.010

Smith, F.E. and T.A. Murphy. 1993. Analysis of rumen ammonia and blood urea nitrogen. https://animalscience.unl.edu/Research/RumNut/RumNutLab/96-bloodureanitrogen .pdf
Van Soest, P.J., J.B. Robertson, and B.A. Lewis. 1991. Methods fordietary fiber, neutral detergent fiber and non-starch polysaccharides (NSP) in relation to animal nutrition. Journal of Dairy Science, v. 74, p.3583-3597, 1991. https://doi.org/10.3168/ jds.S0022-0302(91)78551-2
Wang, C.J., S.P. Wang, H. hou. 2009. Influences of flavomycin, ropadiar, and saponin on nutrient digestibility, rumen fermentation, and methane emission from sheep. Anim. Feed Sci. and Technol. 148 (2–4): 157-166.
Wang, J.K., J.A. Ye, J.X. Liu. 2012. Effects of tea saponins on rumen microbiota, rumen fermentation, methane production and growth performance a review. Tropical Anim Health Prod 44:697-706. https://doi.org/ 10.1007/s11250-011-9960-8
Yildiz, G., A. Tekeli, W. Drochner, and H. Steingass. 2015. Determination of the effects of some plant extracts on rumen fermentation and protozoal counts by hohenheim in vitro gas production Technique. Internat. J. of Anim. and Veterinary Advances 7: 18-26.
Wiswata, I.G.N.A., I.K. Widnyana, and B.P. Udiyana. 2016. Study on Cattle Fodder Avaibility to Support The Development of Balinese Cattle in Bali. Proceeding 2nd International Conference on Sustainability Development Global Sustainable Development. 28 Feb-01 March 2015, Bali-Indonesia. https://unmas.ac.id/wp-content/uploads/2016/10/2nd-ICSD-Proceedings-fix.pdf